Technology Platforms
Genetically Modified Animal
Comprehensive Solutions for Genetically Modified Animals
Genetically modified animal models are useful tools in translational research, particularly for evaluating drug efficacy and elucidating disease mechanisms.
We specialize in the rapid generation and breeding of genetically modified mice and rats to accelerate your drug discovery programs. Our custom-designed models support critical research including target identification and validation, biomarker discovery, and disease model development.
Leveraging homologous recombination using embryonic stem (ES) cells and CRISPR/Cas9 genome editing technology, we efficiently create unique gene knockout (KO) and knock-in (KI) models—delivering results with speed and precision.
Our comprehensive platform with genetically modified (GM) animal models supports drug discovery from various perspectives, such as phenotype characterization, pathological assessment, and pharmacological evaluation, enabling data-driven decision making in significantly shorter timelines.
Human Gene Knock-in Mice and Rats with Exon-Intron Structures
The rise of nucleic acid therapeutics and other modalities targeting human genome sequences has led to a growing demand for humanized knock-in (KI) animal models that faithfully reproduce exon-intron structures found in the human genome. We address this need by generating human gene KI mice using both CRISPR/Cas9 genome editing and homologous recombination with embryonic stem (ES) cells.
For mice, CRISPR /Cas9 enables the insertion of sequences up to approximately 17 kbp, allowing for the rapid generation of models with multi-exon or partial full-length human genes. For larger and more complex insertions—such as full-length genes exceeding this size—we employ ES cell-based technologies, which allow for high-fidelity integration of DNA fragments up to 40 kbp.
For rats, we similarly utilize CRISPR/Cas9-mediated knock-in strategies, achieving insertions up to around 13 kbp, which supports a wide range of human gene integration projects in this species as well.
These advanced models enable in vivo analysis of animal phenotypes and drug efficacy using humanized sequences. We provide flexible support tailored to your nucleic acid and gene therapy drug discovery research needs.
Please consult us if you require:
- Knock-in long DNA inserts for nucleic acid drug evaluation
- Knockout multiple related genes simultaneously
- Modify existing disease models
- Use many genetically modified animals for analysis in a short period
Track record of KI/KO animal creation (FY2017-2024)
| KI mice: 86 | KI rats: 6 | KO mice: 64 | KO rats: 11 |
Rapid Provision of GM Animals for Large-Scale Testing
We accelerate your research by supplying a sufficient numbers of genetically modified mice and rats for large-scale studies in a short timeframe. Utilizing advanced genome editing and in vitro fertilization (IVF) technologies, we streamline both gene modification and breeding processes and significantly reduce study timelines. For example, we can deliver over 20 homozygous knockout (KO) mice or rats within approximately three months from the start of production. The generation and delivery of homozygous knock-in (KI) mice typically require 9 to 10 months from the start of production. For established lines, our optimized approach enables the generation of more than 50 homozygous KO or KI mice within approximately six months. We also provide comprehensive support for sperm and embryo cryopreservation and re-derivation, ensuring continuity and flexibility in maintaining or restarting valuable genetic lines in your research workflows.